Compositions, systems and methods for CRISPR-based enzyme optimization

A method for assessing enzymatic activity, the method comprising:

• combining a reactant barcoded oligonucleotide (RBO) construct with an amplification construct, a blocking construct, and an enzyme in a reaction mixture, wherein the reactant barcoded oligonucleotide (RBO) construct comprises:
• a first oligonucleotide comprising a reaction barcode sequence;
• and a first linker that connects the first oligonucleotide to a first reactant;
• wherein the amplification construct comprises:
• a second oligonucleotide that is complementary to at least a portion of the first oligonucleotide;
• and
• a second linker that connects the second oligonucleotide to a second reactant;
• and
• wherein the blocking construct comprises:
• a third oligonucleotide that is complementary to at least a portion of the first oligonucleotide;
• enzymatically reacting the first reactant with the second reactant to produce a reaction product;
• restricting movement of the amplification construct relative to the RBO construct;
• and
• generating a reverse complement of the reaction barcode sequence with an amplification enzyme.