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US 12,686,883 B2Granted
Sampling method
AssigneeTerumo Kabushiki Kaisha
InventorMasatsugu Igarashi
Life of this patent
Estimating the term…
Abstract
A sampling method includes: a sampling step of circulating a sample from a cell culture device to a sampling channel and measuring concentrations of predetermined components in the sample by a second sensor and a gas concentration sensor; a cleaning step of circulating a cleaning liquid through the sampling channel to circulate the cleaning liquid through the second sensor and the gas concentration sensor after the sampling step; and an air introduction step of replacing the cleaning liquid remaining in the gas concentration sensor with air after the cleaning step.
Claims20
A sampling method comprising:
- a sampling step that includes
- circulating a sample received from a cell culture device to and through a sampling channel of a sampling system, the sampling channel including a biosensor and a gas concentration sensor, the biosensor and the gas concentration sensor each configured to make contact with the sample, and
- measuring concentrations of predetermined components in the sample using the biosensor and the gas concentration sensor;
- a cleaning step that follows the sampling step, the cleaning step including
- circulating a cleaning liquid from a first introduction path of the sampling system through the sampling channel, the cleaning liquid contacting the biosensor and the gas concentration sensor, the first introduction path connecting to the sampling channel upstream of the biosensor and the gas concentration sensor relative to sample flow through the sampling channel;
- and
- an air introduction step that follows the cleaning step, the air introduction step including
- replacing any amount of the cleaning liquid remaining in the gas concentration sensor with air from a second introduction path of the sampling system, the second introduction path being separate from the first introduction path, the second introduction path connecting to the sampling channel downstream from a first connection portion that joins the first introduction path and the sampling channel and upstream of the biosensor and the gas concentration sensor relative to sample flow through the sampling channel,
- wherein the sampling step is performed two or more times, and the second and subsequent sampling steps are performed after the air introduction step.
6 more elements
A sampling method comprising:
- circulating a sample received from a cell culture device to and through a sampling channel of a sampling system, the sampling channel including a biosensor and a gas concentration sensor, the biosensor and the gas concentration sensor each configured to make contact with the sample;
- measuring concentrations of predetermined components in the sample using the biosensor and the gas concentration sensor;
- after the measuring, circulating a cleaning liquid from a first introduction path of the sampling system through the sampling channel, the cleaning liquid contacting the biosensor and the gas concentration sensor, the first introduction path connecting to the sampling channel upstream of the biosensor and the gas concentration sensor relative to sample flow through the sampling channel;
- and
- after the circulation of the cleaning liquid, replacing any amounts of the cleaning liquid remaining in the gas concentration sensor with air from a second introduction path of the sampling sample, the second introduction path being separate from the first introduction path, the second introduction path connecting to the sampling channel downstream from a first connection portion that joins the first introduction path and the sampling channel and upstream of the biosensor and the gas concentration sensor relative to sample flow through the sampling channel.
2 more elements
A sampling method comprising:
- receiving a sample from a cell culture device in a sampling channel of a sampling system, the sampling channel including a biosensor and a gas concentration sensor, the biosensor and the gas concentration sensor each configured to make contact with the sample;
- measuring concentrations of predetermined components in the sample using the biosensor and the gas concentration sensor;
- after the measuring, receiving a cleaning liquid in the sampling channel from a first introduction path of the sampling system, the cleaning liquid contacting the biosensor and the gas concentration sensor, the first introduction path connecting to the sampling channel upstream of the biosensor and the gas concentration sensor relative to sample flow through the sampling channel;
- and
- after the receipt of the cleaning liquid, receiving air in the sampling channel from a second introduction path of the sampling system to replace any amounts of the cleaning liquid remaining in the gas concentration sensor, the second introduction path being separate from the first introduction path, the second introduction path connecting to the sampling channel downstream from a first connection portion that joins the first introduction path and the sampling channel and upstream of the biosensor and the gas concentration sensor relative to sample flow through the sampling channel.
2 more elements
Dependencies are inferred from the claim text.