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US 12,686,880 B2Granted
Methods for increasing mannose content of recombinant proteins
AssigneeAmgen Inc.
InventorsJian Wu, Sean Davern, Simina Crina Petrovan, Michael Charles Brandenstein, Katherine Rose Lindahl, Shawn Erik Lillie
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Abstract
The present invention relates to methods of modulating the mannose content of recombinant proteins.
Claims20
A method of modulating the amount of the mannose-5 glycoform of an Immunoglobulin G (IgG) molecule in an IgG composition, wherein said IgG composition is produced by a Chinese Hamster Ovary (CHO) cell culture, comprising:
- (a) establishing a cell culture of CHO cells in a bioreactor, wherein the CHO cell produces an IgG molecule;
- (b) limiting the amount of glucose in the bioreactor, wherein the concentration of the glucose in the spent medium of the bioreactor is from about 0 to 3 g/L;
- (c) supplementing the cell culture with a feeding medium comprising galactose, such that the concentration of galactose in the resulting spent medium of the bioreactor is above 2.5 g/L;
- and
- (d) harvesting and purifying the IgG composition from the cell culture.
2 more elements
A method of producing an immunoglobulin G (IgG) composition by a Chinese Hamster Ovary (CHO) cell culture, comprising:
- (a) establishing a cell culture of CHO cells in a bioreactor, wherein the CHO cell produces an IgG molecule;
- (b) limiting the amount of glucose in the bioreactor, wherein the concentration of the glucose in the spent medium of the bioreactor is from about 0 to 3 g/L;
- (c) supplementing the cell culture with a feeding medium comprising galactose, such that the concentration of galactose in the resulting spent medium of the bioreactor is above 2.5 g/L;
- and
- (d) harvesting and purifying the IgG composition from the cell culture.
2 more elements
Dependencies are inferred from the claim text.