Method for producing olivetolic acid in an amoebozoa host species
AssigneeLeibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI)
InventorsFalk Hillmann, Christin Reimer, Johann Elias Kufs, Vito Valiante
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Abstract
The present invention relates to a method for the recombinant production of olivetolic acid (OA) in a host species selected from amoebozoa, based on a hybrid-gene or enzyme of polyketide synthase 37 (PKS37) in which the C-terminal type III PKS domain from an amoeba is replaced by an olivetol synthase (OLS) from a plant, and is expressed together with an olivetolic acid cyclase from a multi-gene expression vector. Further provided is a recombinant amoebozoa host species, and an improved method for producing Δ9-tetrahydrocannabinol (THC) or other cannabinoids.
Claims11
A hybrid protein comprising a polyketide synthase 37 (PKS37) having β-ketoacyl synthase activity and lacking a functional C-terminal type III PKS domain, and the hybrid protein comprising a functional plant olivetol synthase (OLS) or an active fragment thereof with olivetol synthase activity at C-terminus of the hybrid protein.
Dependencies are inferred from the claim text.