Soluble ACE2 protein, fusion proteins thereof with an FC domain, and methods of treatment of an ACE2-related disease
AssigneesHuahui Health Ltd., National Institute of Biological Sciences, Beijing
InventorsWenhui Li, Yonghe Qi, Jianhe Chen, Jianhua Sui, Juan Liu, Fengfeng Mao, Ximing Liu
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Abstract
A soluble ACE2 and a truncated form thereof, a fusion protein thereof and preparation methods therefor. A soluble ACE2 and a truncated form thereof, as well as a use of the fusion protein in the preparation of a drug for an ACE2-related disease.
Claims13
An Fc fusion protein multimer ACE2-hFc(n) comprising n polypeptide monomer units, wherein each of the polypeptide monomer units is a dimer in which two soluble angiotensin-converting enzyme 2 (ACE2) truncated forms are linked to N-terminal ends of two heavy chain Fc domains of an antibody respectively,
- wherein said n polypeptide monomer units are further assembled into the Fc fusion protein multimer ACE2-hFc(n) via a tail located at each C-terminal end of the two heavy chain Fc domains,
- wherein each of the two ACE2 truncated forms comprises an extracellular domain of ACE2, and comprises the amino acid sequence as shown by SEQ ID NO:
- 1 or SEQ ID NO:
- 2,
- wherein the tail is an IgM derived tail and comprises the sequence as shown by SEQ ID NO:
- 17,
- wherein each of the two heavy chain Fc domains has a hinge region at its N-terminal, a CH2 domain and a CH3 domain of the antibody, wherein the antibody is an IgG1 antibody, and
- wherein n is selected from 4, 5 or 6.
Dependencies are inferred from the claim text.