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US 12,686,857 B2Granted
Dnase variants
AssigneeNovozymes A/S
InventorsLars Beier, Klaus Gori, Lars Henrik Oestergaard, Lars Giger, Annette Helle Johansen, Gernot J. Abel
Life of this patent
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Abstract
The present invention relates to polypeptide variants and methods for obtaining variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Claims13
A polypeptide variant having DNase activity, which comprises at least five substitutions selected from the group consisting of:
- T1I or T1V,
- G4K,
- S7G,
- K8R,
- S9I,
- S13Y,
- T22P,
- S25P,
- S27L or S27R,
- D32I, D32L, D32R, or D32V,
- L33R or L33K,
- S39P or S39C,
- G41P,
- S42G, S42H, or S42C,
- Q48D,
- D561 or D56L,
- S57W,
- S59V or S59C,
- N61E,
- T65L, T65V, T65M, or T65W,
- S66M, S66W, S66Y, or S66V,
- V76L,
- T77Y,
- F78L,
- P91L,
- S101N,
- S106L, S106R, or S106H,
- Q109R,
- A112E,
- S116D,
- T127V,
- S130A or S130Y,
- T138D,
- Q140V or Q140G,
- S144P,
- A147H,
- C148A,
- W154Y,
- T157V,
- Y159A or Y159R,
- K160V,
- G162S, G162D, or G162C,
- Q166D,
- S167L,
- Q174N,
- G175D or G175N,
- L177Y,
- N178D or N178E,
- S179L,
- C180A, and
- S181E or S181L,
- wherein
- each position corresponds to the position of the polypeptide of SEQ ID NO:
- 1, and
- the polypeptide variant has at least 85% sequence identity to the amino acid sequence of SEQ ID NO:
- 1.
53 more elements
Dependencies are inferred from the claim text.