Culture media based on protein hydrolysate and a process for preparing thereof

A culture medium for the cultivation of cells comprising:

• a protein hydrolysate as a source of amino acids,
• wherein the protein hydrolysate is prepared by an enzymatic hydrolysis of a source protein substrate,
• wherein the cells are non-human metazoan cells,
• wherein a total input of supplemented amino acids, added to the culture medium separately from the protein hydrolysate, is in a range of 0.1 g/L to 10 g/L.

A culture medium for the cultivation of cells,

• comprising a protein hydrolysate as a source of amino acids,
• wherein the protein hydrolysate is prepared by an enzymatic hydrolysis of a protein substrate,
• wherein the cells are non-human metazoan cells,
• wherein a total input of protein hydrolysate, expressed as dry protein weight introduced into the culture medium is in a range of 8 g/L to 50 g/L.

A method for preparing a culture medium for the cultivation of cells comprising:

• comprising providing a protein hydrolysate as a source of amino acids, wherein the protein hydrolysate is prepared by enzymatic hydrolysis of a protein substrate, adding the protein hydrolysate to the culture medium in an amount, expressed as dry protein weight, in a range of 8 g/L to 50 g/L;
• and
• wherein a total input of amino acids from the protein hydrolysate, including amino acids in the form of peptides, bioavailable derivatives, or a combination thereof, is at least 75% by weight of a total input of all amino acids in the culture medium;
• wherein the cells are non-human metazoan cells.