End-to-end cell therapy bioprocessing device for continuous-flow enrichment, washing, and electrotransfection of target cells

A system, comprising:

• an inlet channel that receives a target fluid flow comprising target particles;
• an acoustophoresis device that receives the target fluid flow comprising the target particles from the inlet channel and moves the target particles from the target fluid flow to a buffer fluid flow comprising cargo particles;
• an electroporation device downstream from the acoustophoresis device that receives the buffer fluid flow comprising the target particles and the cargo particles, and applies an electric field to the buffer fluid flow to cause a portion of the target particles in the buffer fluid flow to absorb a portion of the cargo particles;
• a conductivity sensor coupled to an inlet of the electroporation device and configured to measure a conductivity of the buffer fluid flow received by the electroporation device and provide a signal indicating the conductivity;
• an outlet channel that provides an output buffer fluid flow from the electroporation device;
• and
• a controller configured to receive the signal from the conductivity sensor and control a pump to adjust a flow rate of the target fluid flow into the inlet channel based on the conductivity.