Methods for purifying antibodies

A method of purifying a recombinant polypeptide from Host Cell Proteins (HCP), the method comprising:

• (a) applying a solution comprising the recombinant polypeptide and HCP to a superantigen chromatography solid support,
• (b) washing the superantigen chromatography solid support with a wash buffer comprising about 100 mM to about 300 mM caprylate, about 0.75 M to about 1.5 M arginine, about 1 mM to about 500 mM Tris base and about 1 mM to about 500 mM acetic acid, wherein the pH of the wash buffer is about 7.5 to about 8.5;
• and
• (c) eluting the recombinant polypeptide from the superantigen chromatography solid support,
• wherein the superantigen chromatography solid support is a chromatography solid support having a superantigen immobilized therein, and wherein the superantigen is selected from the group consisting of Protein A, Protein G, and Protein L.