Methods and compositions for improving plant traits
AssigneePivot Bio, Inc.
InventorsKarsten Temme, Alvin Tamsir, Sarah Bloch, Rosemary Clark, Emily Tung, Kevin Hammill, Douglas Higgins, Austin Davis-Richardson
Life of this patent
Estimating the term…
Abstract
Methods and systems are provided for generating and utilizing a bacterial composition that comprises at least one genetically engineered bacterial strain that fixes atmospheric nitrogen in an agricultural system that has been fertilized with more than 20 lbs of Nitrogen per acre.
Claims38
A method for increasing the available nitrogen for a non-leguminous plant in a field, said method comprising applying to said non-leguminous plant in said field a plurality of engineered diazotrophic bacteria, wherein:
- said engineered diazotrophic bacteria have a product of (i) colonization ability under field conditions and (ii) fixed N produced per bacterial cell per hour of at least about 2.5×10−8 mmol N per gram of fresh weight of plant root tissue per hour;
- and
- said engineered diazotrophic bacteria comprises one or more genetic variations, wherein the one or more genetic variations comprise a complete or partial nifL deletion and insertion of a constitutive promoter operably linked to a nifA gene.
A non-leguminous plant colonized with a plurality of engineered diazotrophic bacteria, wherein:
- said engineered diazotrophic bacteria have a product of (i) colonization ability under field conditions and (ii) N produced per bacterial cell per hour of at least about 2.5×10−8 mmol N per gram of fresh weight of plant root tissue per hour;
- and
- said engineered diazotrophic bacteria comprise one or more genetic variations, wherein the one or more genetic variations comprise a complete or partial nifL deletion and insertion of a constitutive promoter operably linked to a nifA gene.
A composition comprising:
- a) a plurality of engineered diazotrophic bacteria, wherein:
- said engineered diazotrophic bacteria have a product of (i) colonization ability under field conditions and (ii) N produced per bacterial cell per hour of at least about 2.5×10−8 mmol N per gram of fresh weight of non-leguminous plant root tissue per hour;
- and
- said engineered diazotrophic bacteria comprise one or more genetic variations, wherein the one or more genetic variations comprise a complete or partial nifL deletion and insertion of a constitutive promoter operably linked to a nifA gene;
- and
- b) a non-leguminous plant seed, wherein said plurality of engineered diazotrophic bacteria can provide at least one pound of nitrogen per acre per season to a non-leguminous plant produced from said plant seed in a field.
Dependencies are inferred from the claim text.