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US 12,685,324 B2Granted
Crystallisation of beta-lactoglobulin using multiple protein feeds
AssigneeArla Foods Amba
InventorsKasper Bøgelund Lauridsen, Hans Bertelsen, Nikolaj Drachmann
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Abstract
The present invention relates to an improved method of producing beta-lactoglobulin (BLG) compositions by crystallisation of BLG. The invention makes use of at least two different BLG-containing protein feeds which are mixed to prepare a supersaturated protein solution in which BLG is crystallized. One of the protein feeds must have a pH of at least 5.6 and one of the protein feeds must have a pH of at most 5.4.
Claims26
A method of preparing an edible composition comprising beta-lactoglobulin (BLG), the method comprising the steps of:
- a) preparing an initial protein solution comprising BLG, wherein said initial protein solution is supersaturated with respect to BLG and has a pH in the range of 5-6,
- b) crystallising BLG in the supersaturated, initial protein solution in salting-in mode, thereby obtaining a BLG crystal-containing solution, and
- c) separating BLG crystals from the remaining liquid of the BLG crystal-containing solution,
- wherein step a) involves preparing the initial protein solution by mixing one or more type A protein feed(s) with one or more type B protein feed(s),
- and wherein:
- a “type A protein feed” is defined as a protein feed which comprises BLG and has a pH of at least 5.6, and
- a “type B protein feed” is defined as a protein feed which comprises BLG and has a pH of at most 5.4.
4 more elements
Dependencies are inferred from the claim text.