Hydrophobicity shifted enzyme
AssigneeWaters Technologies Corporation
InventorsMatthew Lauber, Wenjing Li
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Abstract
The present invention relates to a method for analyzing a sample, the method comprising: (a) incubating a sample comprising an analyte with at least one enzyme to produce a digestion mixture comprising fragments of the analyte; (b) loading the digestion mixture onto a reversed-phase chromatography column; and (c) performing reversed-phase chromatography on the digestion mixture, wherein the at least one enzyme is hydrophobically modified to increase a retention time of the at least one enzyme such that the at least one enzyme elutes from the reversed-phase chromatography column later than the fragments of the analyte.
Claims8
A method for analyzing a sample, the method comprising:
- (a) incubating a sample comprising an analyte with hydrophobically modified trypsin to produce a digestion mixture comprising fragments of the analyte;
- (b) loading the digestion mixture onto a reversed-phase chromatography column;
- and
- (c) performing reversed-phase chromatography on the digestion mixture, wherein the hydrophobically modified trypsin is hydrophobically modified at the C-terminus with a sequence extension comprising at least three hydrophobic amino acid residues to increase a retention time of the hydrophobically modified trypsin such that the hydrophobically modified trypsin elutes from the reversed-phase chromatography column later than the fragments of the analyte.
Dependencies are inferred from the claim text.