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US 12,681,009 B2Granted
Molecular target in cancer
AssigneeThe Regents of the University of California
InventorTannishtha Reya
Life of this patent
Estimating the term…
Abstract
Disclosed are methods for identifying small molecule modulators of protein-RNA interactions that could potentially function as therapeutic agents in cancer treatment. Methods of identifying inhibitors of the RNA-binding functions of MSI2, as well as inhibitors identified thereof, are also disclosed.
Claims12
A method of identifying a modulator of protein-RNA interaction comprising:
- (a) contacting a fluorophore-labeled RNA-binding protein (RBP) with a fluorophore-labeled target RNA in a reaction mix, (b) detecting a first fluorescence signal generated by the interaction of the RBP and the target RNA, (c) introducing an agent into the reaction mix, (d) detecting a second fluorescence signal in the presence of the agent, wherein the agent is an inhibitor of the RBP-RNA interaction if the second fluorescence signal is decreased relative to the first fluorescence signal, and wherein the agent is an enhancer of the RBP-RNA interaction if the second fluorescence signal is increased relative to the first fluorescence signal, wherein the RBP is U1A and the fluorophore-labeled target RNA comprises AUUGCACUCCUUUUA-Biotin (SEQ ID NO:
- 2).
Dependencies are inferred from the claim text.