Method of bioanalytical analysis utilizing ion spectrometry, including mass analysis

A method of bioanalytical analysis utilizing ion spectrometry, including mass analysis, comprising:

• providing a pooled or otherwise composite sample which combines a plurality of individual samples, each individual sample containing a multitude of analyte compounds;
• subjecting an aliquot of pooled or otherwise composite sample to a first ion spectrometric measurement cycle, which disperses the analyte compounds according to a first number of physical-chemical properties, and obtaining a first spectral dataset of the thusly dispersed analyte compounds;
• evaluating the first spectral dataset and obtaining a first table of annotated features therefrom;
• subjecting an aliquot of one or more individual samples from the plurality of individual samples to a second ion spectrometric measurement cycle individually, the second ion spectrometric measurement cycle dispersing the analyte compounds according to a second number of physical-chemical properties, being smaller than the first number of physical-chemical properties, and obtaining a second spectral dataset of the thusly dispersed analyte compounds for each of the one or more individual samples;
• evaluating the second spectral dataset and obtaining a second table of annotated features therefrom for each of the one or more individual samples, wherein the evaluating and obtaining includes at least one of using, applying, referring to, and calling on the first table of annotated features;
• and
• using at least one of the first table of annotated features and the second table of annotated features for one or more of the individual samples for characterizing one or more of the analyte compounds.