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US 12,680,959 B2Granted
Siderophore-based biosensors for iron detection
AssigneeSaudi Arabian Oil Company
InventorsAbdulmohsen A. Al-Humam, Nora K. Al-Sudairi, Manar A. AlAhmari
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Abstract
This disclosure relates to methods of detecting iron in a water injection system, such as a water injection system used in the oil and gas industry, using an optical siderophore-based biosensor. This disclosure also relates to methods of detecting corrosion using an optical siderophore-based biosensor.
Claims22
A method for detecting a presence of iron in a water injection system, the method comprising:
- contacting a biosensor with a sample of water from the water injection system, wherein the biosensor comprises:
- an iron chelator produced by a bacteria, wherein the iron chelator transduces a detectable signal;
- and
- a permeable matrix entrapping or encapsulating the iron chelator;
- measuring a signal from the biosensor, wherein a signal transduced by the iron chelator when the iron chelator is bound to iron differs from a signal transduced by the iron chelator when the iron chelator is not bound to iron;
- and
- comparing the signal to a control value, wherein a difference in signal indicates the presence of iron in the sample;
- wherein the water injection system is part of an oil and gas pipeline, oil and gas storage tank, or oil and gas vessel.
5 more elements
A method for detecting corrosion in a water injection system, the method comprising:
- contacting a biosensor with a sample of water from the water injection system, wherein the biosensor comprises:
- an iron chelator produced by a bacteria, wherein the iron chelator transduces a detectable signal;
- and
- a permeable matrix entrapping or encapsulating the iron chelator;
- measuring a signal from the biosensor, wherein a signal transduced by the iron chelator when the iron chelator is bound to iron differs from a signal transduced by the iron chelator when the iron chelator is not bound to iron;
- and
- comparing the signal to a control value, wherein a difference in signal indicates the presence of iron in the sample;
- wherein the water injection system is part of an oil and gas pipeline, oil and gas storage tank, or oil and gas vessel, and wherein the presence of iron in the sample indicates corrosion in the water injection system.
5 more elements
A method for detecting a presence of iron in a water injection system, the method comprising:
- contacting a biosensor with a sample of water from the water injection system, wherein the biosensor comprises:
- a fluorescent siderophore produced by a bacteria selected from the group consisting of Pseudomonas aeruginosa, Pseudomonas oryzihabitans, Pseudomonas stutzeri, Pseudomonas putida, Pseudomonas fluorescens, Azotobacter vinelandii, Acinetobacter baumannii, and Paracoccus denitrificans, wherein the fluorescent siderophore is an iron chelator;
- and
- a permeable matrix entrapping or encapsulating the fluorescent siderophore;
- measuring a signal from the biosensor, wherein a signal transduced by the fluorescent siderophore when the fluorescent siderophore is bound to iron differs from a signal transduced by the fluorescent siderophore when the fluorescent siderophore is not bound to iron;
- and
- comparing the signal to a control value, wherein a difference in signal indicates the presence of iron in the sample;
- wherein the water injection system is part of an oil and gas pipeline, oil and gas storage tank, or oil and gas vessel.
5 more elements
Dependencies are inferred from the claim text.