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US 12,680,924 B2Granted
Device used for real time field sampling for DNA extraction
OwnerRobert G Saundersinventor
Life of this patent
Estimating the term…
Abstract
A sampling device including an intake pump coupled with a volume controller to provide a known volume of samples such as liquid collected through a specialized hollow membrane filter. Once the known volume of sample is filtered the intake pump is turned off, an air pump turns on and removes residual or excess sample. The membrane filter is then preserved for extraction of DNA/RNA.
Claims20
A device comprising:
- at least one or more membrane filters, wherein each of said at least one or more membrane filters is operable for collecting one or more samples for extraction of DNA/RNA, each of said at least one or more membrane filters comprises a plurality of hollow membrane tubes or filaments being operable for filtering;
- one or more filter holders, said one or more filter holders are configured to engage said at least one or more membrane filters, wherein each of said at least one or more membrane filters having a unique serial number for identification;
- at least one intake pump, wherein said at least one intake pump is operable for suctioning and/or transporting said one or more samples to said at least one or more membrane filters for sample collection;
- a volume controller, said volume controller is configured to control a flow of said one or more samples being transported to said one or more membrane filters, each having a unique serial number for identification for said sample collection;
- wherein said volume controller determines an amount or volume of said one or more samples flowing to said one or more membrane filters;
- wherein said volume controller is operable for turning said intake pump off and to turn off the flow of one or more samples, once a preprogrammed level of said one or more samples is reached;
- wherein each of said at least one or more membrane filters are preserved for extraction of DNA/RNA;
- wherein each of said at least one or more membrane filters further comprises a first female Luer lock connector that is configured to be operable for receiving input from said volume controller;
- wherein each of said at least one or more membrane filters further comprises a second female Luer lock connector that is configured to be operable for engaging with one or more waste lines;
- and
- an air pump coupled to an air pump switch, said air pump comprising an air compressor.
8 more elements
A sampling device consisting of:
- one or more sample collecting means for collecting one or more samples for extraction of DNA/RNA, each of said one or more sample collecting means comprises a plurality of hollow membrane tubes or filaments configured to be operable for filtering;
- means for engaging said one or more sample collecting means, wherein each of said one or more sample collecting means further comprising a unique serial number for identification;
- means for pumping or suctioning the one or more samples to said one or more sample collecting means;
- means for controlling a flow of the one or more samples, the one or more samples being transported to said one or more sample collecting means;
- means for determining an amount or volume of the one or more samples flowing to said one or more sample collecting means;
- means for turning an intake pump off and to turn off the flow of one or more samples, once a preprogrammed level of the one or more samples is reached;
- means for preserving the DNA/RNA in the one or more samples;
- means for controlling an operation of at least one of said volume determining means and flow controlling means;
- means for priming said pumping or suctioning means and discharge waste prior to sampling;
- means for purging out residual samples from said one or more sample collection means;
- and
- means for avoiding contamination of one or more collected samples.
9 more elements
A device comprising:
- at least one or more membrane filters, wherein each of said at least one or more membrane filters is configured to be operable for collecting one or more samples for extraction of DNA/RNA, each of said at least one or more membrane filters comprises a plurality of hollow membrane tubes or filaments being operable for filtering;
- one or more filter holders, said one or more filter holders are configured to engage said at least one or more membrane filters, wherein each of said at least one or more membrane filters having a unique serial number for identification;
- at least one intake pump, wherein said at least one intake pump is operable for suctioning and/or transporting said one or more samples to said at least one or more membrane filters for sample collection;
- a volume controller, said volume controller is configured to control a flow of said one or more samples being transported to said one or more membrane filters;
- a priming valve, said priming valve is configured to be operable for allowing said intake pump to prime and to discharge sample waste prior to sampling;
- wherein said volume controller determines an amount or volume of said one or more samples flowing to said one or more membrane filters;
- wherein said volume controller is operable for turning said intake pump off and to turn off the flow of samples, once a preprogrammed level of said one or more samples is reached;
- wherein said at least one or more membrane filters are preserved for extraction of DNA/RNA;
- and
- an air pump, said air pump comprising an air compressor, wherein said air pump is configured to purge out residual samples from said one or more membrane filters.
7 more elements
Dependencies are inferred from the claim text.