Spatial-dependent analysis of biological material from intact tissue samples
AssigneeGenentech, Inc.
InventorsSoren Warming, Xin Ye, Jun Zou, Jonas Doerr, Michelle Dourado, Matthew Chang
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Abstract
Biological research requires isolation and analysis of material, for example, RNA, DNA and protein, from tissue samples. The methods and compositions described herein allow for high resolution imaging of large and intact tissue samples, and subsequent isolation of material in a precise and location dependent-manner. The methods and compositions described herein may be used, for example, for biomarker discovery, identification of cell populations, pathology analysis, and generation of expression data in specific regions of interest.
Claims15
A method for 3-dimensional expression profiling of an intact tissue or tissue sample, comprising:
- (a) providing a three-dimensional intact tissue or tissue sample;
- (b) clearing the sample;
- (c) contacting the tissue or tissue sample with a photo-activatable label;
- (d) subjecting a region of the tissue or tissue sample to a multi-photon laser, thereby labeling the region;
- (e) imaging the labeled region to create an image;
- (f) isolating the labeled region from the tissue or tissue sample;
- (g) determining a DNA, RNA, and/or protein composition of the isolated labeled region;
- and
- (h) combining the image with the DNA, RNA, and/or protein composition of the isolated labeled region to create a 3-dimensional expression profile of the intact tissue or tissue sample.
Dependencies are inferred from the claim text.