Spatial transcriptomics in pips
AssigneeIllumina, Inc.
InventorRobert Meltzer
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Abstract
This disclosure provides methods and systems for single-cell, multi-omic analysis of target cells without microfluidic devices. The disclosed methods involve the use of template particles to template the formation of monodisperse droplets to generally capture a single target cell from a population of cells in an encapsulation, derive a plurality of distinct mRNA molecules from the single target cell, and quantify the distinct mRNA molecules to generate an expression profile. Nucleic-acid-tagged antibody conjugates are used for simultaneous proteomic analysis along with the gene expression profiling.
Claims21
A method for gene expression profiling, the method comprising:
- incubating a tissue sample on an array comprising a plurality of labels having binding specificity for a cellular protein and having a nucleic acid index identifying the location of the label on the array, wherein the labels are lyophilized and released upon wetting of the array;
- disaggregating a plurality of cells from the tissue sample;
- combining the disaggregated plurality of cells with template particles;
- and
- generating a plurality of uniform partitions substantially instantly that encapsulate a single one of the template particles and a single one of the disaggregated cells to form pre-templated instant partitions (PIPs);
- and
- releasing in each PIP nucleic acid molecules and proteins from the disaggregated cells, including proteins bound by a label from the array.
Dependencies are inferred from the claim text.