Method for detection of specific nucleic acids

A method for detecting a target RNA polynucleotide, comprising the following steps:

• (i) annealing a catcher polynucleotide to the target RNA polynucleotide to obtain a hybrid of the target RNA polynucleotide and the catcher polynucleotide, wherein the catcher polynucleotide is immobilized on a surface or connected to a bead and comprises a segment that is at least partially complementary to a portion of the target RNA polynucleotide and a poly T-section that is at least partially complementary to a poly-A-tail of the target RNA polynucleotide,
• (ii) annealing a mature crRNA (CRISPR RNA) molecule to a specific target sequence on the target RNA polynucleotide to form a complex;
• (iii) binding a catalytically-inactive crRNA-guided RNA-binding protein to the complex of step (ii) to obtain a protein-RNA polynucleotide complex;
• (iv) removing unbound mature crRNA molecule and unbound catalytically inactive crRNA-guided RNA-binding protein;
• (v) binding an antibody to the protein-RNA polynucleotide complex of step (iii);
• and
• (vi) detecting the antibody-bound protein-RNA polynucleotide complex of step (v).