Hyaluronic acid-producing recombinant cells
AssigneeGivaudan SA
InventorsDominique Louis, Valentina Bevilacqua, Lionel Durant, Karine Jaillardon, Dominique Thomas
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Abstract
The present invention relates to the field of bio-production of hyaluronic acid. There is a need in the art for hyaluronic acid production methods allowing its highly efficient synthesis and secretion. The solution proposed in the present invention is the use of a genetically modified cell comprising many modifications as described in the present text. The present invention further proposes methods allowing the bio-production of hyaluronic acid having a controlled molecular weight using the genetically modified cells of the invention.
Claims17
A recombinant host cell (i) or a Saccharomyces cerevisiae recombinant yeast cell (ii) producing hyaluronic acid (HA) wherein the recombinant host cell (i) or the S. cerevisiae recombinant yeast cell (ii) comprises:
- (a) one or more recombinant nucleic acids encoding a polypeptide having hyaluronan synthase activity;
- (b) one or more recombinant nucleic acids encoding a polypeptide having UDP-Glucose dehydrogenase (UDP-GlcDH or HASB) activity;
- (c) one or more recombinant nucleic acids encoding a polypeptide having hyaluronidase activity wherein the polypeptide having hyaluronidase activity comprises a secretion signal so that hyaluronic acid-is produced by the recombinant host cell (i) or the S. cerevisiae recombinant yeast cell (ii), wherein the hyaluronic acid produced by the recombinant host cell (i) is of a desired molecular weight (HAMW),
- and wherein the polypeptide having hyaluronidase activity comprised in the recombinant host cell (i) further comprises an anchoring signal;
- and
- (d) (i) one or more recombinant nucleic acids encoding a polypeptide having a glutamine synthetase (GLN1) activity;
- and/or
- (ii) one or more disrupted endogeneous nucleic acids encoding a glutamate synthase (GLT1).
Dependencies are inferred from the claim text.