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US 12,680,116 B1Granted
Systems, compositions, and methods of fermentation with Z. mobilis
AssigneePOET Research, Inc.
InventorsStephen M. Lewis, Benjamin P. Gacke, Jennifer Rebecca Headman, Neelakantam V. Narendranath
Life of this patent
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Abstract
Methods, compositions, and systems for propagation and fermentation, particularly large scale operations for production of ethanol and dried distiller's grain are provided. Addition of Z. mobilis to propagation and/or fermentation decreases lactic acid bacterial contamination.
Claims23
A method of fermentation comprising:
- (a) grinding grain to form a ground grain;
- (b) adding water to the ground grain to form an aqueous slurry;
- (c) contacting the slurry with a yeast;
- (d) contacting the slurry with a Z. mobilis prior to step (c) or simultaneously with step (c);
- (e) maintaining the slurry under growth conditions over time;
- and
- (f) recovering one or more products of fermentation;
- wherein the one or more products of fermentation comprise reduced glycerol relative to products generated in a fermentation with the yeast in the absence of Z. mobilis;
- or
- wherein residual starch after fermentation is lower relative to residual starch after fermentation with the Z. mobilis in the absence of yeast.
7 more elements
A method of fermentation comprising:
- (a) grinding grain to form a ground grain;
- (b) adding water to the ground grain to form an aqueous slurry;
- (c) contacting the slurry with a yeast;
- (d) contacting the slurry with a Z. mobilis;
- (e) maintaining the slurry under growth conditions over time;
- and
- (f) recovering one or more products of fermentation;
- wherein during fermentation (i) ethanol concentration rises, (ii) Z. mobilis ethanol production rate decreases to a rate less than yeast ethanol production rate after (i), and (iii) the concentration of ethanol continues to rise after (ii);
- wherein the one or more products of fermentation comprise reduced glycerol relative to products generated in a fermentation with the yeast in the absence of Z. mobilis;
- or
- wherein residual starch after fermentation is lower relative to residual starch after fermentation with the Z. mobilis in the absence of yeast.
8 more elements
Dependencies are inferred from the claim text.