Single cell transfection with interchangeable reagent

A system for single cell transfection comprising:

• a transfection chamber on a transfection chip;
• a fluidic channel located on the transfection chip for guiding a cell towards the transfection chamber, the fluidic channel sized to allow no more than a single cell to travel from a cell reservoir through the fluidic channel to arrive at the transfection chamber at a time;
• a microfluidic ejector;
• a first reagent receiver located on the transfection chip guiding a first received reagent towards the transfection chamber and intersecting with a path of the fluidic channel upstream of the transfection chamber;
• and
• a second reagent receiver located on the transfection chip guiding a second received reagent towards the transfection chamber and intersecting with the path of the fluidic channel upstream of the transfection chamber,
• wherein the first reagent receiver intersects the fluidic channel at a first distance from the transfection chamber and the second reagent receiver intersects the fluidic channel at a second distance from the transfection chamber different from the first distance,
• wherein the transfection chamber comprises a constriction reducing a size of the fluidic channel such that edges of the transfection chamber apply a physical pressure on the cell within the transfection chamber, the physical pressure to porate a cell membrane of the cell to receive the first reagent from the first reagent receiver;
• wherein the transfection chamber comprises a first electroporation element inside the transfection chamber on a first edge of the edges of the transfection chamber, the first edge configured to receive the cell from the fluidic channel, and a second electroporation element inside the transfection chamber on a second edge of the edges of the transfection chamber opposite the first edge of the transfection chamber, the second edge configured to output the cell to the microfluidic ejector.