Gene transfection system and method

A gene transfection system, wherein the system comprises:

• an acoustothermal module and a signal generating module;
• the acoustothermal module comprises:
• a piezoelectric substrate, an acoustothermal chip arranged on the piezoelectric substrate, and N sound-absorbing vessels arranged on the acoustothermal chip and configured for culturing recipient cells, wherein N is an integer greater than 1;
• the signal generating module is configured to output a basic frequency signal;
• the acoustothermal chip is configured to convert the basic frequency signal to an acoustic wave signal, establish a temperature gradient field in the sound-absorbing vessels with the acoustic wave signal, and precisely control the temperature of the recipient cells in the sound-absorbing vessels to a specified temperature that enables a perforation effect on membranes of the recipient cells to reach a level of 100% by controlling an intensity of the acoustic wave signal at a predetermined acoustic wave signal intensity based on the temperature gradient field, so that pores are opened on the membranes of the recipient cells, and nucleic acids or other substances are allowed to go into the recipient cells through these pores;
• wherein the temperature gradient field is a set of temperature values with gradient changes established based on changes of the acoustic wave signal, each temperature value corresponds to an acoustic wave signal strength and also corresponds to a perforation effect on the membranes of the recipient cells in a sound-absorbing vessel;
• wherein the acoustothermal chip comprises M interdigital transducers, a first controller, and a second controller;
• and M is an integer greater than 1;
• the sound-absorbing vessels are made of polydimethylsiloxane (PDMS), and each sound-absorbing vessel contains an independent chamber;
• the interdigital transducer is used to generate a surface acoustic wave based on the basic frequency signal;
• the first controller is used to individually control the finger period in each of the interdigital transducers;
• the second controller is used to periodically activate part or all of the interdigital transducers;
• the first controller and the second controller are further used to construct an array of the interdigital transducers to change the thermal field distribution in the acoustothermal chip.