Method of single strand RNA purification employing an anion exchanger
AssigneeSartorius BIA Separations d.o.o.
InventorPeter S. Gagnon
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Abstract
A method of single strand RNA purification employing an anion exchanger comprising the steps applying a sample containing single-stranded RNA to an anion exchanger, washing the anion exchanger at a first temperature in the range of 18° C. to 25° C. with a first salt solution having a first ionic strength in the range 0.5 M to 12.0 M, eluting the single stranded RNA by a second salt solution having a second ionic strength at a second temperature in the range of 35° C. to 80° C., with the proviso that the first ionic strength is at least 0.5 M higher than the second ionic strength.
Claims17
A method of single strand RNA purification employing an anion exchanger comprising the steps of:
- applying a sample containing single-stranded RNA to an anion exchanger,
- washing the anion exchanger at a first temperature in the range of 18° C. to 25° C. with a first salt solution having a first ionic strength in the range of 0.5 M to 12.0 M,
- eluting the single stranded RNA by a second salt solution having a second ionic strength at a second temperature in the range of 35° C. to 80° C.,
- with the proviso that the first ionic strength is at least 0.5 M higher than the second ionic strength.
Dependencies are inferred from the claim text.