Protease substrate, and polypeptide including protease cleavage sequence
AssigneeChugai Seiyaku Kabushiki Kaisha
InventorsMika Sakurai, Tatsuya Kawa, Hitoshi Katada, Naoka Hironiwa
Life of this patent
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Abstract
The present application relates to protease substrates, peptide sequences cleavable by a protease, polypeptides comprising a protease cleavage sequence and methods for production thereof, pharmaceutical compositions comprising a polypeptide comprising a protease cleavage sequence, and methods for releasing an antigen-binding domain or a ligand by the cleavage of a protease cleavage sequence included in a polypeptide.
Claims16
A protease substrate comprising a sequence selected from the following:
- the sequence spanning from the N-terminal fourth to fifteenth amino acids of a sequence selected from SEQ ID NOs:
- 430, 400, 8, 26, 107, 114, 117, 130, 133, 135, 143, 149, 155, 156, 170, 172, 179, 184, 191, 203, 225, 265, 268, 271, 339, 359, and 425;
- the sequence spanning from the N-terminal fourth to thirteenth amino acids of a sequence selected from SEQ ID NOs:
- 430, 400, 8, 26, 107, 114, 117, 130, 133, 135, 143, 149, 155, 156, 170, 172, 179, 184, 191, 203, 225, 265, 268, 271, 339, 359, and 425;
- or
- the sequence spanning from the N-terminal sixth to thirteenth amino acids of a sequence selected from SEQ ID NOs:
- 430, 400, 8, 26, 107, 114, 117, 130, 133, 135, 143, 149, 155, 156, 170, 172, 179, 184, 191, 203, 225, 265, 268, 271, 339, 359, and 425.
A method for releasing a ligand bound to a ligand-binding molecule, wherein the method comprises cleaving, by protease, a portion contained in the ligand-binding molecule capable of binding to the ligand, wherein the portion has at least one sequence selected from the following:
- the sequence spanning from the N-terminal fourth to fifteenth amino acids of a sequence selected from SEQ ID NOs:
- 430, 400, 8, 26, 107, 114, 117, 130, 133, 135, 143, 149, 155, 156, 170, 172, 179, 184, 191, 203, 225, 265, 268, 271, 339, 359, and 425;
- the sequence spanning from the N-terminal fourth to thirteenth amino acids of a sequence selected from SEQ ID NOs:
- 430, 400, 8, 26, 107, 114, 117, 130, 133, 135, 143, 149, 155, 156, 170, 172, 179, 184, 191, 203, 225, 265, 268, 271, 339, 359, and 425;
- the sequence spanning from the N-terminal sixth to thirteenth amino acids of a sequence selected from SEQ ID NOs:
- 430, 400, 8, 26, 107, 114, 117, 130, 133, 135, 143, 149, 155, 156, 170, 172, 179, 184, 191, 203, 225, 265, 268, 271, 339, 359, and 425.
Dependencies are inferred from the claim text.