Fungal cellulase variants with improved stability
AssigneeAB Enzymes Finland Oy
InventorsKari Juntunen, Leena Valtakari, Susanna Mäkinen, Marika Alapuranen, Hendrik Hellmuth, Maike Anders, Marc Mennicken, Ulrich Schwaneberg, David Schönauer, Pentti Ojapalo, Terhi Puranen
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Abstract
The invention relates to novel variants of fungal endoglucanases. The invention further relates to enzyme preparations and detergent compositions comprising the variant as well as to processes for treating cellulosic material with the variant. The variants have depilling, antipilling and/or antigreying performance, and improved stability in the presence of proteases.
Claims29
A variant of a GH45 cellulase polypeptide, wherein the variant comprises a substitution Q167Y and substitutions in amino acid positions 210, 215, 220 and 225, wherein the amino acid positions are numbered with reference to a parent GH45 cellulase having an amino acid sequence set forth in SEQ ID NO:
- 1;
- wherein the variant has at least 95% sequence identity with the parent GH45 cellulase of SEQ ID NO:
- 1;
- and wherein the variant has improved protease stability in comparison to the parent GH45 cellulase as determined a after storage for three days at 20-22° C. in a liquid detergent comprising 0.7% (w/w) protease.
A method for treating a textile material, wherein the method comprises contacting the textile material with a detergent composition comprising a detergent and a variant of a GH45 cellulase polypeptide, wherein the variant comprises a substitution Q167Y and substitutions in amino acid positions 210, 215, 220 and 225, wherein the amino acid positions are numbered with reference to a parent GH45 cellulase having an amino acid sequence set forth in SEQ ID NO:
- 1;
- wherein the variant has at least 95% sequence identity with the parent GH45 cellulase of SEQ ID NO:
- 1;
- and wherein the variant has improved protease stability in comparison to the parent GH45 cellulase as determined after storage for three days at 20-22° C. in a liquid detergent comprising 0.7% (w/w) protease.
Dependencies are inferred from the claim text.