Engineered lipase variants
AssigneeSociete des Produits Nestle S.A.
InventorsChinping Chng, William Casey Hallows, Judy Victoria Antonio Viduya, Nikki Dellas, Stephanie Sue Galanie, Kristen Jean Vallieu
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Abstract
Engineered lipase polypeptides have been optimized to provide improved thermostability, protease stability, and stability under a range of pH conditions, including acidic (pH<7) and basic (pH>7) conditions. Compositions containing the engineered lipase polypeptides can be used for therapeutic and/or nutritional purposes. Polynucleotides encoding the engineered lipase polypeptides are also provided, as well as methods for making the engineered polynucleotides and lipase polypeptides.
Claims20
A method comprising culturing a host cell to produce at least one recombinant lipase, the host cell comprising an expression vector comprising at least one recombinant polynucleotide sequence encoding the at least one recombinant lipase, the at least one recombinant lipase comprising an amino acid sequence comprising at least 70% sequence identity to SEQ ID NO:
- 2 and at least 80% sequence identity to SEQ ID NO:
- 868 and further comprising at least a substitution at position 149, 161, 174, 175, 189, 225, 271, or 293, or combinations thereof with reference to SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.