Administration of kynurenine depleting enzymes for tumor therapy
AssigneeBoard of Regents, The University of Texas System
InventorsGeorge Georgiou, Everett Stone
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Abstract
Methods and compositions related to the use of a protein with kynureninase activity are described. For example, in certain aspects there may be disclosed a modified kynureninase capable of degrading kynurenine. Furthermore, certain aspects of the invention provide compositions and methods for the treatment of cancer with kynurenine depletion using the disclosed proteins or nucleic acids.
Claims18
A plasmid comprising a nucleic acid that encodes a polypeptide comprising a kynureninase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 8, and having one or more substitutions relative to SEQ ID NO:
- 8, wherein the one or more substitutions comprise a substitution of the Phe found at position 306 of SEQ ID NO:
- 8.
A pharmaceutical formulation comprising:
- (i) a plasmid comprising a nucleic acid that encodes a polypeptide comprising a kynureninase enzyme, wherein the kynureninase enzyme comprises an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 8 and has one or more substitutions relative to SEQ ID NO:
- 8, wherein the one or more substitutions comprise a substitution of the Phe found at position 306 of SEQ ID NO:
- 8;
- and
- (ii) a pharmaceutically acceptable carrier.
A method of producing a polypeptide comprising a kynureninase enzyme, wherein the kynureninase enzyme comprises an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 8 and has one or more substitutions relative to SEQ ID NO:
- 8, wherein the one or more substitutions comprise a substitution of the Phe found at position 306 of SEQ ID NO:
- 8, the method comprising:
- (i) providing a plasmid comprising a nucleic acid that encodes the polypeptide;
- (ii) introducing the plasmid into a host cell under conditions sufficient for the host cell to express the polypeptide;
- and
- (iii) isolating the polypeptide from the host cell.
Dependencies are inferred from the claim text.