Mutated PGLB oligosaccharyltransferase enzymes
AssigneeGlaxosmithkline Biologicals SA
InventorsJoel Melby, Joel David Karpiak, Stefan Jochen Kemmler, Deepak Rudrappa, Laura Elizabeth Yates
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Abstract
The present disclosure provides mutated PglB oligosaccharyltransferase enzymes, polynucleotides that encode the mutated PglB oligosaccharyltransferase enzymes, host cells capable of expressing the engineered PglB oligosaccharyltransferase enzymes, N-glycosylated proteins that are made using the engineered PglB oligosaccharyltransferase enzymes, and methods of using the engineered PglB oligosaccharyltransferase enzymes.
Claims15
A PglB oligosaccharyltransferase (OST) polypeptide comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 2 comprising:
- (a) at least one substituted residue at an amino acid selected from the group consisting of X477, X273, X65, X69, X72, X78, X101, X125, X147, X151, X176, X195, X199, X202, X204, X205, X206, X223, X260, X272, X275, X279, X281, X282, X284, X289, X293, X303, X305, X306, X310, X315, X320, X358, X360, X362, X371, X382, X397, X402, X462, X466, X489, X520, X523, X570, and X605, and wherein the substituted residue is different from the amino acid at the corresponding position in SEQ ID NO:
- 2;
- and
- (b) amino acid 273 of SEQ ID NO:
- 2 is mutated to a residue selected from the group consisting of L, D, E, K, and R.
Dependencies are inferred from the claim text.