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US 12,680,086 B2Granted
Modified beta-1,3-n-acetylglucosaminyltransferase polypeptides
AssigneeAmyris, Inc.
InventorsJessica Walter, Wenzong Li, Christopher F. Mugler, Victoria Hsiao
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Abstract
Provided herein are variant β-1,3-N-acetylglucosaminyltransferase polypeptides capable of producing lacto-n-neotetraose, yeast cells capable of producing one or more human milk oligosaccharides, and methods of making such cells. Also, provided are fermentation compositions including the disclosed genetically modified yeast cells, and related methods of producing and recovering HMOs generated by the yeast cells.
Claims20
A variant β-1,3-N-acetylglucosaminyltransferase (LgtA) polypeptide comprising a G179R or G179K amino acid substitution relative to the amino acid sequence of SEQ ID NO:
- 1, wherein the polypeptide has an amino acid sequence that is from 90% to 99.7% identical to the amino acid sequence of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.