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US 12,680,079 B2Granted
System and method for extraction and cryopreservation of bone marrow
AssigneeOssium Health, Inc.
InventorsErik J. Woods, Brian H. Johnstone, Dongsheng Gu, Aubrey Marie Sherry, Kelsey Gwen Musall, John R. Woods, James Hardin, Alan Hooks
Life of this patent
Estimating the term…
Abstract
Methods are provided for extracting bone marrow cells from bone obtained from deceased donors, for preparing the bone marrow for cryopreservation and for obtaining desired cells from cryopreserved and fresh bone marrow.
Claims21
A method for recovering transplantable stem cells, the method comprising:
- (a) obtaining cadaver bone or cadaver bone fragments that have been surface sterilized;
- (b) preparing ground cadaver bone from the surface sterilized cadaver bone or cadaver bone fragments and processing the ground cadaver bone in the presence of a grinding medium thereby extracting, at least, fat and bone marrow cells and obtaining a fatty emulsion comprising the extracted fat and bone marrow cells;
- (c) placing the fatty emulsion in a centrifuge container capable of being centrifuged;
- (d) centrifuging the centrifuge container under conditions such that bone marrow cells are concentrated or pelleted towards the bottom of the centrifuge container and a fat layer is formed towards the top of the centrifuge container;
- and
- (e) draining the concentrated or pelleted bone marrow cells from the bottom of the centrifuge container while the fat layer remains in the centrifuge container, thereby collecting the extracted bone marrow cells and recovering transplantable stem cells,
- wherein between steps (d) and (e) a bag clamp or a bag clip is placed on the centrifuge container just below the fat layer thereby physically separating the fat layer from the concentrated or pelleted bone marrow cells and preventing passage of the fat layer during step (e).
4 more elements
A method for recovering transplantable stem cells, the method comprising:
- (a) obtaining cadaver bone or cadaver bone fragments that have been surface sterilized;
- (b) preparing ground cadaver bone from the surface sterilized cadaver bone or cadaver bone fragments and processing the ground cadaver bone in the presence of a grinding medium thereby extracting, at least, fat and bone barrow cells and obtaining a fatty emulsion comprising extracted fat and bone marrow cells;
- (c) filtering out the processed ground cadaver bone;
- (d) placing the fatty emulsion in a centrifuge container capable of being centrifuged;
- (e) centrifuging the centrifuge container under conditions such that bone marrow cells are concentrated or pelleted towards the bottom of the centrifuge container and a fat layer is formed towards the top of the centrifuge container;
- and
- (f) draining the concentrated or pelleted bone marrow cells from the bottom of the centrifuge container while the fat layer remains in the centrifuge container, thereby collecting the extracted bone marrow cells and recovering transplantable stem cells,
- wherein between steps (e) and (f) a bag clamp or a bag clip is placed on the centrifuge container adjacent to and just below the fat layer thereby physically separating the fat layer from the concentrated or pelleted bone marrow cells and preventing passage of the fat layer during step (f).
5 more elements
Dependencies are inferred from the claim text.