Antigen presenting T cells, sensitized, manufactured T cells and methods of treatment using the same

A method for treating a solid tumor in a subject comprising:

• obtaining a sample of immune cells harvested from the subject, wherein the sample of immune cells comprises T cells;
• adding the sample of immune cells to a culture medium at a cell density of at least 1.5×106 cells per mL, wherein the culture medium comprises temsirolimus and an IL-2 signaling inhibitor, wherein said temsirolimus is present in said culture medium at a concentration of at least 1 UM;
• incubating said sample of immune cells in the culture medium for a first period of time, wherein said first period of time is at least 16 hours;
• adding IFN-α, IL-7, and IL-15 to the culture medium after the first period of time, wherein said IFN-α is added to said culture medium to a concentration of at least 10,000 IU/mL;
• incubating said sample of immune cells in the culture medium for a second period of time following addition of IFN-α, IL-7, and IL-15 to yield manufactured T cells, wherein IL-2 is not added to the culture medium at any time during the production of manufactured T cells, and wherein no anti-CD3/anti-CD28 co-stimulation is performed at any time during the method production of manufactured T cells;
• and
• harvesting said manufactured T cells, wherein 5% or less of the manufactured T cells that are CD4+ or CD8+ express CD25 as measured by flow cytometry;
• and
• administering a therapeutically effective amount of the manufactured T cells to the subject.