Cassette assembly

A cassette assembly comprising:

• a cover;
• a first cassette half;
• a second cassette half;
• and
• a slider, wherein
• the cover is configured to be positioned onto the first cassette half and the second cassette half and keep the first cassette half and second cassette half together with the slider sandwiched between the first cassette half and the second cassette half;
• the slider comprises N≥2 test chambers in the form of through holes through the slider;
• each of the first cassette half and the second cassette half comprises:
• N waste tanks connected in series and separated by respective walls;
• and
• an excessive liquid tank in fluid connection with the waste tanks by a vertical outlet channel and by an air valve;
• an inlet port of the first cassette half is configured to receive a liquid to sequentially fill each waste tank and a head space defined above the waste tanks;
• and
• the air valves are configured to be opened to drain liquid in the head spaces into the excessive liquid tanks through the vertical outlet channels and to form a separation of equal volumes of liquid in the waste tanks;
• wherein the slider comprises a first through hole in connection with a first end of the slider and a second through hole in connection with a second end of the slider;
• each of the first cassette half and the second cassette half comprises:
• a circumferential channel having a first opening configured to be aligned with the first through hole and a second opening configured to be aligned with the second through hole;
• a serpentine channel interconnecting the circumferential channel and the waste tanks;
• the inlet port of the first cassette half is in fluid connection with the circumferential channel;
• and
• the serpentine channels are configured to present a higher flow resistance to the liquid as compared to the circumferential channels to enable filling of the circumferential channels prior to entering of the liquid into the waste tanks through the serpentine channels.

A cassette assembly comprising:

• a cover;
• a first cassette half;
• a second cassette half;
• and
• a slider, wherein
• the cover is configured to be positioned onto the first cassette half and the second cassette half and keep the first cassette half and second cassette half together with the slider sandwiched between the first cassette half and the second cassette half;
• the slider comprises N≥2 test chambers in the form of through holes through the slider;
• each of the first cassette half and the second cassette half comprises:
• N waste tanks connected in series and separated by respective walls;
• and
• an excessive liquid tank in fluid connection with the waste tanks by a vertical outlet channel and by an air valve;
• an inlet port of the first cassette half is configured to receive a liquid to sequentially fill each waste tank and a head space defined above the waste tanks;
• and
• the air valves are configured to be opened to drain liquid in the head spaces into the excessive liquid tanks through the vertical outlet channels and to form a separation of equal volumes of liquid in the waste tanks;
• wherein the slider is movable relative to the first cassette half and the second cassette half between a sample filling position and a flow position;
• one of the first cassette half and the second cassette half comprises an inlet port configured to receive a biological sample comprising a gel suspension that can be polymerized into three dimensional (3D) culture matrices in the test chambers in the slider when the slider is in the sample filling position;
• and
• each of the first cassette half and the second cassette half comprises a channel system forming, together with the test chambers in the slider, a meander pattern and configured to sequentially fill the test chambers with the biological sample received at the inlet port.