Method for producing cell population containing CAR-expressing immune cells
AssigneesShinshu University, Kyoto Prefectural Public University Corporation, BrightPath Biotherapeutics Co., Ltd.
InventorsYozo Nakazawa, Shigeki Yagyu, Miyuki Tanaka, Kayoko Nakamura, Masahiro Okada, Makoto Kondo, Tomokuni Shigeura, Shogo Hirota
Life of this patent
Estimating the term…
Abstract
The present disclosure includes a method of producing a cell population containing Chimeric Antigen Receptor (CAR)-expressing immune cells, comprising co-culturing CAR-expressing immune cells and cells expressing a target antigen of the CAR, wherein the CAR-expressing immune cells are cells into which a CAR gene has been introduced and the target antigen-expressing cells are normal blood cells that have been engineered to express the target antigen.
Claims17
A method of producing a cell population containing Chimeric Antigen Receptor (CAR)-expressing immune cells, comprising co-culturing CAR-expressing immune cells and cells expressing a target antigen of the CAR, wherein the CAR-expressing immune cells are cells into which a CAR gene has been introduced and the target antigen-expressing cells are cells that have been prepared by gene transfer into peripheral blood mononuclear cells (PBMCs), wherein the target antigen is Human epidermal growth factor receptor 2 (HER2) or Ephrin type-B receptor 4 (EPHB4).
Dependencies are inferred from the claim text.