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US 12,679,889 B2Granted
Oxyntomodulin binding molecules, and uses thereof
AssigneeEli Lilly and Company
InventorsRobert John Konrad, Wenyu Ming, Robert William Siegel, II, Tara Suzanne Umberger
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Abstract
Provided herein are novel N- and C-terminal oxyntomodulin polypeptide binding molecules, and uses thereof, including a method for quantitating oxyntomodulin.
Claims26
A polypeptide molecule selected from the group consisting of
- (a) a polypeptide molecule that binds the N-terminal region of each of human oxyntomodulin (SEQ ID NO:
- 25) and human glucagon (SEQ ID NO:
- 30), wherein the polypeptide molecule comprises the complementarity determining regions (CDRs) set forth in SEQ ID NOS:
- 1-6 and
- (b) a polypeptide molecule that binds the C-terminal region in each of human oxyntomodulin (SEQ ID NO:
- 25) and human glicentin (SEQ ID NO:
- 28), wherein the polypeptide molecule comprises the complementarity determining regions (CDRs) set forth in SEQ ID NOS:
- 13-18.
5 more elements
A nucleic acid molecule selected from the group consisting of
- (a) a nucleic acid molecule comprising one or both of a first nucleic acid sequence encoding SEQ ID NO:
- 9 and a second nucleic acid sequence encoding SEQ ID NO:
- 10 and
- (b) a nucleic acid molecule comprising one or both of a first nucleic acid sequence encoding SEQ ID NO:
- 21 and a second nucleic acid sequence encoding SEQ ID NO:
- 22.
3 more elements
A vector selected from the group consisting of
- (a) a vector comprising one or both of a first nucleic acid sequence encoding SEQ ID NO:
- 9 and a second nucleic acid sequence encoding SEQ ID NO:
- 10 and
- (b) a vector comprising one or both of a first nucleic acid sequence encoding SEQ ID NO:
- 21 and a second nucleic acid sequence encoding SEQ ID NO:
- 22.
3 more elements
A process of producing a polypeptide molecule, comprising culturing a cell comprising
- (a) one or both of a first nucleic acid sequence encoding SEQ ID NO:
- 9 and a second nucleic acid sequence encoding SEQ ID NO:
- 10 or
- (b) one or both of a first nucleic acid sequence encoding SEQ ID NO:
- 21 and a second nucleic acid sequence encoding SEQ ID NO:
- 22,
- under conditions such that the polypeptide molecule is expressed, and recovering the expressed polypeptide molecule from the culture medium.
4 more elements
A method of determining the concentration of human oxyntomodulin (SEQ ID NO:
- 25) in a liquid sample, comprising:
- (a) contacting a liquid sample comprising human oxyntomodulin with a first polypeptide molecule that binds the C-terminal region in each of human oxyntomodulin (SEQ ID NO:
- 25) and human glicentin (SEQ ID NO:
- 28), wherein the first polypeptide molecule comprises the CDRs of SEQ ID NOS:
- 13-18, thereby forming a first polypeptide molecule-human oxyntomodulin complex;
- (b) contacting the first polypeptide molecule-human oxyntomodulin complex with a second polypeptide molecule that binds the N-terminal region of each of human oxyntomodulin (SEQ ID NO:
- 25) and human glucagon (SEQ ID NO:
- 30), wherein the second polypeptide molecule comprises the CDRs of SEQ ID NOS:
- 1-6, thereby forming a first polypeptide molecule-human oxyntomodulin-second polypeptide molecule complex;
- and
- (c) quantitating the amount of oxyntomodulin in the first polypeptide molecule-human oxyntomodulin-second polypeptide molecule complex by comparison against a standard curve of known amounts of human oxyntomodulin (SEQ ID NO:
- 25).
9 more elements
Dependencies are inferred from the claim text.