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US 12,679,873 B2Granted
Alkali-tolerant affinity chromatography ligands
AssigneeGuangzhou Binding Technologies Co., Limited
InventorYinong Zong
Life of this patent
Estimating the term…
Abstract
This disclosure relates to chromatography ligands comprising one or more domains of Staphylococcal Protein A (SPA), or any functional variants thereof, as well as methods of making and using the same.
Claims16
A polypeptide comprising a first immunoglobulin (Ig)-binding domain, wherein the first Ig-binding domain comprises:
- (1) the amino acid sequence that is identical to SEQ ID NO:
- 1, except that the amino acid that corresponds to N55 of SEQ ID NO:
- 1 is Val or Leu and the amino acid that corresponds to S44 of SEQ ID NO:
- 1 is Ala, Val, Ile, Leu, Met, Phe, Tyr, or Trp;
- (2) the amino acid sequence that is identical to SEQ ID NO:
- 2, except that the amino acid that corresponds to N54 of SEQ ID NO:
- 2 is Val or Leu and the amino acid that corresponds to S43 of SEQ ID NO:
- 2 is Ala, Val, Ile, Leu, Met, Phe, Tyr, or Trp;
- (3) the amino acid sequence that is identical to SEQ ID NO:
- 3, except that the amino acid that corresponds to N52 of SEQ ID NO:
- 3 is Val or Leu and the amino acid that corresponds to S41 of SEQ ID NO:
- 3 is Ala, Val, Ile, Leu, Met, Phe, Tyr, or Trp;
- (4) the amino acid sequence that is identical to SEQ ID NO:
- 4, except that the amino acid that corresponds to N52 of SEQ ID NO:
- 4 is Val or Leu and the amino acid that corresponds to S41 of SEQ ID NO:
- 4 is Ala, Val, Ile, Leu, Met, Phe, Tyr, or Trp;
- (5) the amino acid sequence that is identical to SEQ ID NO:
- 5, except that the amino acid that corresponds to N52 of SEQ ID NO:
- 5 is Val or Leu and the amino acid that corresponds to S41 of SEQ ID NO:
- 5 is Ala, Val, Ile, Leu, Met, Phe, Tyr, or Trp;
- or
- (6) the amino acid sequence that is identical to SEQ ID NO:
- 6, except that the amino acid that corresponds to N52 of SEQ ID NO:
- 6 is Val or Leu and the amino acid that corresponds to S41 of SEQ ID NO:
- 6 is Ala, Val, Ile, Leu, Met, Phe, Tyr, or Trp.
22 more elements
A polypeptide comprising an immunoglobulin (Ig)-binding domain, wherein the Ig-binding domain comprises SEQ ID NO:
- 6 with a mutation at N52, wherein N52 is substituted with a Val or Leu amino acid residue and wherein the Ig-binding domain further comprises a mutation at S41, wherein S41 is substituted with a hydrophobic amino acid residue selected from Ala, Val, Ile, Leu, Met, Phe, Tyr, or Trp.
Dependencies are inferred from the claim text.