Fluidic detection and control algorithm for PCR analysis
AssigneeFormulatrix International Holding Ltd.
InventorsRasmus Lindblom, Michael Nilsson
Life of this patent
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Abstract
Disclosed are various embodiments for systems and methods for detecting and making real-time adjustments to positional control of a fluidic volume moving through an optical detection region in a fluidic channel of a chip on a RT-qPCR system. Said embodiments are disclosed within a RT-qPCR system having a chip and a cartridge for receiving the chip, and further a mechanical instrument for interacting with a membrane of the chip for positional control. Further, example embodiments include systems and methods for capacitive detection of a fluid within a fluidic channel as well as optical detection within an optical detection region of a fluidic channel within a chip.
Claims21
A system for detecting and making real-time adjustments to positional control of a fluidic volume moving through an optical detection region in a fluidic channel, comprising:
- a mechanical instrument;
- a chip, comprising:
- (i) a fluidic channel, wherein the mechanical instrument controls the motion of a fluidic volume;
- and
- (ii) an optical detection region;
- one or more independent heat blocks, wherein the chip is positioned at least partially on the one or more independent heat blocks so that the fluidic channel is within close proximity to the one or more independent heat blocks;
- an optical detection unit, comprising:
- (i) an optical light-emitting element;
- (ii) an optical detector;
- and
- (iii) a processing unit for performing analysis on the optical detection region of the chip, comprising detecting the presence of a sample and signal output of the sample within the optical detection region, and wherein the processing unit adjusts a motion control script based on at least the signal output, wherein the motion control script adjusts at least a start and/or stop of the mechanical instrument.
A method for detecting and making real-time adjustments to positional control of a fluidic volume moving through an optical detection region in a fluidic channel, comprising:
- preparing a sample with a fluorescent marker;
- configuring a chip to receive the sample, wherein the chip has a fluid channel for the sample to flow along, and an optical detection region that allows for optical light transmission to the fluid channel;
- applying the sample to the chip;
- initiating an optical detection unit, wherein initiating the optical detection unit is configured to illuminate one or more LED's and to activate an optical detection diode;
- applying the chip to two heating arrays;
- initiating a mechanical instrument to depress regions of the chip, wherein the depressed regions cause the fluid within the chip, including the sample, to move along the fluidic channel and across the two heating arrays;
- detecting, by the optical detection unit, presence of the sample and signal output of the sample within the optical detection region;
- and
- adjusting a motion control script based on at least the signal output, wherein the motion control script adjusts at least a start and/or stop of the mechanical instrument.
A method for detecting and making real-time adjustments to positional control of a fluidic volume moving in a fluidic channel, comprising:
- preparing a sample with magnetic beads that attract nucleic acid compounds;
- configuring a chip to receive the sample, wherein the chip has a fluid channel for the sample to flow along, and the fluid channel crosses two separate heating arrays;
- applying the sample to the chip;
- applying a capacitive array along sections of the fluid channel of the chip;
- initiating a mechanical instrument to depress regions of the chip, wherein the depressed regions cause the fluid within the chip, including the sample, to move along the fluidic channel and across the two separate heating arrays;
- and
- detecting, by capacitive sensors, capacitance change in the fluid channel at the capacitive array.
Dependencies are inferred from the claim text.