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US 12,678,493 B2Granted
Compositions and methods for multiplexed tumor vaccination with endogenous gene activation
AssigneeYale University
InventorsSidi Chen, Ryan D. Chow, Guangchuan Wang
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Abstract
The present invention includes compositions and methods for treating or preventing cancer. Embodiments include cell-based and viral vector-based vaccines that utilize gene expression activation systems to augment the product of endogenous genes to treat or prevent cancer.
Claims7
A composition comprising a cancer vaccine, comprising a modified cell comprising a CRISPRa system capable of increasing expression of a plurality of endogenous human antigen presentation genes resulting in the modified cell expressing at least one tumor associated antigen (TAA),
- wherein the CRISPRa system comprises an sgRNA library,
- wherein the plurality of endogenous human antigen presentation genes are selected from the group consisting of CD70, CD80, CD86, IFNA4, IFNB1, and IFNG;
- and
- wherein the sgRNA library comprises every nucleic acid sequence of SEQ ID NOs:
- 1, 2, 5, 6, 9, 11-13, 16, 18, 21, 22, 24, 25, 28, 30, 31, and 33-35.
2 more elements
Dependencies are inferred from the claim text.