Polypeptide applied to inhibition of intracellular lipid accumulation and synthesis method thereof

A synthesis method of a polypeptide comprising SEQ ID NO:

• 1 applied to an inhibition of an intracellular lipid accumulation, comprising the following steps:
• 1) Soaking a resin in an organic solution, washing off the organic solution from the resin, removing a protecting group on the resin with a deprotecting agent to obtain a first resulting resin, and washing the first resulting resin;
• 2) Taking a second amino acid at a free carboxyl terminal and adding a condensing agent and N,N-dimethylformamide (DMF) into the first resulting resin for a reaction to obtain a second resulting resin, and then taking the second resulting resin for a first color testing;
• 3) Removing a protecting group on the second amino acid with the deprotecting agent to obtain a resulting product, and washing the resulting product;
• 4) Repeating step 2) and step 3) for a connection of amino acids until a last amino acid is connected;
• 5) Removing a protecting group on the last amino acid with the deprotecting agent to obtain a third resulting resin, washing the third resulting resin and then subjecting the third resulting resin to a second color testing;
• and
• 6) After a completion of the reaction, washing and shrinking a polypeptide product with a solution, digesting the polypeptide product by a lysis buffer to obtain a digested polypeptide of SEQ ID NO:
• 1, and finally, analyzing and purifying the digested polypeptide.