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US 12,678,464 B2Granted
Methods for stem cell transplantation
AssigneeThe UAB Research Foundation
InventorsLawrence S Lamb, Shin Mineishi, Ayman Saad
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Abstract
The present disclosure provides methods of hematopoietic stem cell transplantation (HSCT). In particular, the present disclosure provides a method of HSCT using a combination of an in-vivo T-cell depletion method, with an ex-vivo method of γδ T cell expansion and as T cell depletion. The in-vivo T-cell depletion method depletes (in-vivo) the alloreactive T cells that would otherwise increase the risk of GvHD.
Claims16
A method for allogeneic hematopoietic stem cell transplantation (HSCT) in a subject suffering from a hematologic malignancy comprising the steps of:
- (a) administering to the subject on day 0 an allogeneic hematopoietic stem cell first graft infusion, wherein the allogenic hematopoietic stem cells are haploidentical and minimally manipulated;
- (b) After day 0, administering to the subject an agent which provides in vivo T cell depletion, wherein the agent is cyclophosphamide;
- and
- (c) After administering the agent which provides in vivo T cell depletion, administering to the subject a γδ T cell product, wherein the γδ cell product comprises an expanded population of γδ T cells and is depleted in αβ T cells, and further wherein the γδ cell product comprises greater than or equal to 60% γδ T cells, less than or equal to 5% αβ T cells, and less than or equal to 25% NK cells as determined by flow cytometric analysis;
- wherein the method further comprises administering a preparatory chemotherapy regimen prior to day 0, and
- wherein the method reduces the risk of relapse of the hematologic malignancy.
3 more elements
Dependencies are inferred from the claim text.