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US 12,677,851 B2Granted
Methods for detoxifying bran and products obtained therefrom
AssigneeThe Florida International University Board of Trustees
InventorsBarry P. Rosen, Jian Chen
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Abstract
The subject invention provides a bran composition comprising a bran component with a reduced level of inorganic arsenic, a grain product that comprises such bran composition and at least one other component and/or other ingredient, and food products that are made from such bran composition and/or such grain product. The subject invention also provides methods of detoxifying a bran and/or grain product.
Claims19
A method of detoxifying a bran composition, comprising the steps of:
- (1) Creating a slurry by suspending the bran composition in a buffer solution, the bran composition having at least 1 mg/kg of inorganic arsenic by weight;
- (2) Methylating inorganic arsenic in the slurry by treating the slurry with a methylating enzyme;
- and
- (3) determining arsenic species and level in the treated slurry, more than 90% of the inorganic arsenic present in the bran composition being converted into dimethylarsenate (DMA).
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A method of detoxifying a grain product, comprising the steps of:
- mixing the grain product with a buffer solution to create a slurry, the grain product comprising a bran composition having at least 1 mg/kg of inorganic arsenic by weight;
- adding an arsenic S-adenosylmethionine methyltransferase in the slurry and incubating the slurry and the arsenic S-adenosylmethionine methyltransferase at a temperature of about 50° C.-80° C. to methylate the inorganic arsenic;
- determining arsenic species and level in the mixture of the slurry and the arsenic S-adenosylmethionine methyltransferase, more than 90% of the inorganic arsenic present in the bran composition being converted into DMA, and
- optionally, drying the mixture of the slurry and the arsenic S-adenosylmethionine methyltransferase.
1 more element
Dependencies are inferred from the claim text.