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US 12,674,799 B2Granted
Cell-free expression of antibodies, antigen-binding fragments thereof, and antibody derivatives
AssigneeNorthwestern University
InventorsBastian Vogeli, Michael Christopher Jewett, Andrew Colin Hunt
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Abstract
The present technology relates to cell-free systems, methods, and kits for expressing proteins in vitro and evaluating the expressed proteins. In particular, the technology relates to cell-free systems, methods, and kits for expressing antibodies, antigen-binding fragment thereof, and antibody derivatives in vitro and evaluating the expressed antibodies, antigen-binding fragment thereof, and antibody derivatives.
Claims20
A method comprising:
- a) assembling a linear expression template (LET) from nucleic acid sequences, wherein assembling does not include the use of cells or cell culture;
- and wherein assembling comprises Golden Gate assembly or Gibson assembly;
- b) amplifying the LET to generate amplification products;
- c) contacting the amplification products of (b) with a cell-free protein expression system (CFPS) to produce protein products;
- d) characterizing the protein products of (c), wherein characterization comprises Amplified Luminescent Proximity Homogeneous Linked Immunosorbent Assay (AlphaLISA).
2 more elements
Dependencies are inferred from the claim text.