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US 12,674,795 B2Granted
Method for peritoneal metastatic cell detection and isolation thereof
AssigneeVersitech Limited
InventorsSze Tsai Alice Wong, Ho Cheung Anderson Shum, Shanshan Li, Ka Man Ip, Kei Shuen Tang
Life of this patent
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Abstract
Provided herein is a method for the detection and isolation of metastatic cells from a sample obtained from a subject. Also provided is a device comprising a microchip for use in the method. Also provided is a method of isolating the metastatic cells for culture and characterization. Provided herein is a kit for the method therein.
Claims13
A method for detecting peritoneal metastatic cells in a sample obtained from a subject comprising:
- (a) flowing the sample using a microfluidic device at an effective wall shear stress, wherein a channel bottom of the microfluidic device is coated with P-selectin, wherein the effective wall shear stress is about 0.1-0.15 dyne/cm2, and contacting peritoneal metastatic cells and peritoneal non-metastatic cells in the sample with P-selectin such that the peritoneal non-metastatic cells are all washed away, and the peritoneal metastatic cells selectively bind to the P-selectin;
- and (b) detecting selective binding of the peritoneal metastatic cells to the P-selectin.
Dependencies are inferred from the claim text.