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US 12,674,778 B2Granted
Graphene-enabled DNA biosensors with enhanced sensitivity and specificity
AssigneeThe Trustees of the University of Pennsylvania
InventorsAlan T. Johnson, Jr., Zhaoli Gao, Han Xia
Life of this patent
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Abstract
Provided are electronic biosensors based on graphene field effect transistors that utilize engineered hairpin probe DNA that allows for target recycling and hybridization chain reactions.
Claims20
A detector, comprising:
- a first portion of functionalized graphene;
- a first hairpin probe (H1) comprising a plurality of nucleotides, the first hairpin probe (H1) being linked through a graphene field effect transistor (GFET) to the first portion of functionalized graphene,
- in its hairpin configuration, the first hairpin probe (H1) comprising (a) a bottom portion linked to the portion of graphene, and (b) a paired portion, and
- the first hairpin probe (H1) opening upon binding by a target molecule (T) with the paired portion of the hairpin probe (H1), the binding giving rise to an H1-T complex having a paired region formed between the first hairpin probe (H1) and the target (T),
- the H1-T complex further having a protruding segment.
2 more elements
A method of detecting a target, comprising
- to a first hairpin probe (H1) comprising a plurality of nucleotides, the first hairpin probe (H1) being linked through a graphene field effect transistor (GFET) to a first portion of graphene, in its hairpin configuration, the first hairpin probe (H1) comprising (a) a bottom portion linked to the portion of graphene, (b) a paired portion,
- contacting the first hairpin probe (H1) with a target molecule (T) under such conditions that the target molecule (T) binds with the paired portion of the hairpin probe (H1) and the first hairpin probe (H1) opens, the binding giving rise to an H1-T complex having a paired region formed between the first hairpin probe (H1) and the target (T), the H1-T complex further having a protruding segment.
A method of detecting a target, comprising:
- to one or more first hairpin probes (H1i=1 to n) each comprising a different plurality of nucleotides,
- the plurality of first hairpin probes being linked through a graphene field effect transistor (GFET) to and a first portion of graphene,
- each of the plurality of first hairpin probes in its hairpin configuration comprising (a) a bottom portion linked to the portion of graphene, (b) a paired portion, and
- contacting the plurality of first hairpin probes with one or more target molecules (Tj, j=1 to m) under such conditions that one or more of the target molecules binds with the paired portion of one or more first hairpin probes and the first hairpin probe opens,
- the binding giving rise to an H1i-Tj complex having a paired region formed between the first hairpin probe (Hi1) and the target (Tj), the H1i-T complex further having a protruding segment.
2 more elements
Dependencies are inferred from the claim text.