US 12,674,754 B2 · Method for detecting target biomolecule in biological sample, disposable optical fiber sensor therefor, and cartridge thereof

Abstract

Disclosed are: a method for detecting a target biomolecule in a biological sample, in which the method can be rapidly and inexpensively performed on site to diagnose diseases such as infectious diseases; an FO-LSPR optical fiber sensor which can be used therein; and a disposable sensor cartridge having the sensor mounted. The disposal sensor cartridge of the present invention can be used in the rapid detection of a target biomolecule, by mounting an optical fiber sensor and then connecting FO-LSPR equipment thereto. When an FO-LSPR molecular diagnostic system using the optical fiber sensor cartridge of the present invention is combined with a CRISPR-Cas system, target biomolecules can be detected accurately and conveniently without nucleic acid amplification.

Claims5

Dependent claims

A method for detecting a target nucleic acid in a test sample, comprising:

a) contacting a CRISPR RNA, which binds to a target nucleic acid, and a Cas protein, with a test sample isolated from a living body so that the CRISPR RNA and the Cas protein are allowed to react with the test sample to obtain a reaction product;
b) contacting the reaction product with a fiber optics-localized surface plasmon resonance (FO-LSPR) sensor containing metal nanoparticles having nucleic acid oligomers attached thereto;
and
c) irradiating the sensor with light to measure a reflective index intensity change value of an FO-LSPR output signal to detect the presence of the target nucleic acid in the test sample.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

18/021,434Filed 25 Aug 2021

Priority

KR 10-2020-010833627 Aug 2020

Published

US 2024/0035968 A11 Feb 2024

Granted

7 Jul 2026

Term ends

Not estimatedThe source records don’t support an estimate.

Classification13 codes

G01N 21/554
And using surface plasmons: detecting the surface plasmon resonance of nanostructured metals, e.g. localised surface plasmon resonance
C12N 9/22
Acting on ester bonds: ribonucleases [RNase]; Deoxyribonucleases [DNase]
C12N 9/226
Clustered regularly interspaced short palindromic repeats [CRISPR]-associated [CAS] enzymes: class 2 CAS enzyme complex, e.g. single CAS protein
C12N 15/10
Recombinant DNA-technology: processes for the isolation, preparation or purification of DNA or RNA

Method for detecting target biomolecule in biological sample, disposable optical fiber sensor therefor, and cartridge thereof

Life of this patent

Method for detecting target biomolecule in biological sample, disposable optical fiber sensor therefor, and cartridge thereof

Life of this patent

Abstract

Claims5