US 12,674,752 B2 · Rapid detection and identification of bacteria directly from whole blood with light scattering spectroscopy based biosensor

Abstract

Methods for identifying bacterial species in biofluid samples (e.g., whole blood samples) are described. The methods rely on optical spectroscopy, and enable rapid detection and identification of bacteria directly from whole blood. Not only can LSS-based techniques detect and identify bacteria in biofluids such as whole blood, but that species-level identification can potentially be made based on a small number of bacterial cells, without the need for observing entire colonies or performing susceptibility testing. The methods may comprise illuminating the biofluid sample with input light, detecting scattered light produced by the biofluid sample in response to the illuminating, generating first data indicative of a measured scattering spectrum associated with the biofluid sample using the detected scattered light, and identifying whether at least one of the bacterial species is present in the biofluid sample using the first data.

Claims18

Dependent claims

A method for identifying bacterial species in a biofluid sample, comprising:

illuminating the biofluid sample with input light;
detecting scattered light produced by the biofluid sample in response to the illuminating;
generating first data indicative of a measured scattering spectrum associated with the biofluid sample using the detected scattered light;
and
identifying whether at least one of the bacterial species is present in the biofluid sample using the first data, wherein identifying whether at least one of the bacterial species is present in the biofluid sample comprises:
obtaining second data indicative of a plurality of reference scattering spectra, each reference scattering spectrum being associated with a respective one of the bacterial species;
generating third data indicative of a linear combination of the reference scattering spectra, the linear combination comprising a plurality of unknown coefficients representing respective concentrations for the bacterial species in the biofluid sample;
and
determining the unknown coefficients using the first data and the third data.

6 more elements

A system for identifying bacterial species in a biofluid sample, comprising:

a light source;
a spectrograph and a photosensitive detector;
first and second polarizers, the first polarizer being disposed on a first optical path formed between the light source and the biofluid sample and the second polarizer being disposed on a second optical path formed between the biofluid sample and the spectrograph;
and
a processor coupled to the photosensitive detector and configured to:
generate first data indicative of a measured scattering spectrum associated with the biofluid sample;
and
identify whether at least one of the bacterial species is present in the biofluid sample using the first data, wherein the processor is configured to identify whether at least one of the bacterial species is present in the biofluid sample by:
obtaining second data indicative of a plurality of reference scattering spectra, each reference scattering spectrum being associated with a respective one of the bacterial species;
generating third data indicative of a linear combination of the reference scattering spectra, the linear combination comprising a plurality of unknown coefficients representing respective concentrations for the bacterial species in the biofluid sample;
and
determining the unknown coefficients using the first data and the third data.

9 more elements

Dependencies are inferred from the claim text.

Rapid detection and identification of bacteria directly from whole blood with light scattering spectroscopy based biosensor

Life of this patent

Rapid detection and identification of bacteria directly from whole blood with light scattering spectroscopy based biosensor

Life of this patent

Abstract

Claims18