Back to results
US 12,674,745 B2Granted
Sample carrier and method for imaging a sample
AssigneeLeica Microsystems CMS GmbH
InventorsSoeren Alsheimer, Joachim Bradl
Life of this patent
Estimating the term…
Abstract
A sample carrier for receiving a sample includes an optical medium in which the sample is received, the optical medium having a first refractive index. A window portion defining two parallel surfaces includes an optically transparent material having a second refractive index, and is arranged at a bottom side of the sample carrier. The first and second refractive indices do not deviate by more than 2.5%.
Claims20
An imaging system for imaging a sample, comprising:
- a sample carrier for receiving the sample, the sample carrier comprising:
- an optical medium in which the sample is received, the optical medium having a first refractive index;
- and
- a window portion defining two parallel surfaces that comprises an optically transparent material having a second refractive index, and that is arranged at a bottom side of the sample carrier, wherein the first and second refractive indices do not deviate by more than 2.5%;
- a sample carrier receiving portion configured to receive the sample carrier;
- an optical detection system, comprising a first objective arranged below, above or to the side of the sample carrier receiving portion and being configured to receive detection light emitted by the sample;
- and
- a second objective arranged below, above or to the side of the sample carrier receiving portion and configured to receive the detection light emitted by the sample,
- wherein an optical axis of the first objective and the parallel surfaces of the window portion enclose an oblique angle when the sample carrier is received in the sample carrier receiving portion, and
- wherein an optical axis of the second objective and the parallel surfaces of the window portion enclose an oblique angle when the sample carrier is received in the sample carrier receiving portion.
7 more elements
A method for imaging a sample with an imaging system, the method comprising the following steps:
- providing a sample carrier having an optical medium for embedding the sample, the optical medium having a first refractive index, and a window portion defining two parallel surfaces, comprising an optically transparent material that has a second refractive index, and is arranged at a bottom side of the sample carrier, wherein the first and second refractive indices do not deviate by more than 2.5%;
- arranging the sample in the sample carrier by immersing the sample in the optical medium;
- positioning the sample carrier in a sample carrier receiving portion of an imaging device;
- and
- capturing at least one image of the sample with an optical detection system of the imaging device, wherein the optical detection system comprises first objective arranged below, above or to the side of the sample carrier and configured to receive detection light emitted by the sample,
- wherein the optical axis of the first objective and the parallel surfaces of the window portion enclose an oblique angle,
- wherein the imaging system comprises a second objective arranged below, above or to the side of the sample carrier receiving portion and configured to receive the detection light emitted by the sample, and
- wherein the optical axis of the second objective and the parallel surfaces of the window portion enclose an oblique angle when the sample carrier is received in the sample carrier receiving portion.
5 more elements
An imaging system for imaging a sample, comprising:
- a sample carrier for receiving a sample, comprising:
- an optical medium in which the sample is received, the optical medium having a first refractive index;
- and
- a window portion defining two parallel surfaces that comprises an optically transparent material having a second refractive index, and that is arranged at a bottom side of the sample carrier, wherein the first and second refractive indices do not deviate by more than 2.5%;
- a sample carrier receiving portion configured to receive the sample carrier;
- an optical detection system, comprising a first objective arranged below, above or to the side of the sample carrier receiving portion and being configured to receive detection light emitted by the sample, wherein an optical axis of the first objective and the parallel surfaces of the window portion enclose an oblique angle when the sample carrier is received in the sample carrier receiving portion;
- and
- a second objective arranged below, above or to the side of the sample carrier receiving portion and configured to receive the detection the light emitted by the sample, wherein an optical axis of the second objective and the parallel surfaces of the window portion enclose an oblique angle when the sample carrier is received in the sample carrier receiving portion,
- wherein at least one of an illumination characteristic and a detection characteristic are different for the first objective and for the second objective for generating images from different dyes in the sample with the first and the second objective, or
- wherein the optical axis of the first objective encloses an angle with the optical axis of the second objective within a range of 50° to 90°.
7 more elements
A method for imaging a sample with an imaging system, the method comprising the following steps:
- providing a sample carrier having an optical medium for embedding the sample, the optical medium having a first refractive index, and a window portion defining two parallel surfaces, comprising an optically transparent material that has a second refractive index, and is arranged at a bottom side of the sample carrier, wherein the first and second refractive indices do not deviate by more than 2.5%;
- arranging the sample in the sample carrier by immersing the sample in the optical medium;
- positioning the sample carrier in a sample carrier receiving portion of an imaging device;
- and
- capturing at least one image of the sample with an optical detection system of the imaging device, wherein the optical detection system comprises a first objective arranged below, above or to the side of the sample carrier and configured to receive detection light emitted by the sample,
- wherein the optical axis of the first objective and the parallel surfaces of the window portion enclose an oblique angle,
- wherein the imaging system comprises a second objective arranged below, above or to the side of the sample carrier receiving portion and configured to receive the detection light emitted by the sample, and wherein the optical axis of the second objective and the parallel surfaces of the window portion enclose an oblique angle when the sample carrier is received in the sample carrier receiving portion,
- wherein at least one of an illumination characteristic and a detection characteristic are different for the first objective and for the second objective for generating images from different dyes in the sample with the first and the second objective, or
- wherein the optical axis of the first objective encloses an angle with the optical axis of the second objective within a range of 50° to 90°.
6 more elements
Dependencies are inferred from the claim text.