Method and system for detection of particles focused asymmetrically
AssigneeBio-Rad Laboratories, Inc.
InventorAhmadreza Rashedi
Life of this patent
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Abstract
Methods and systems for detecting particles. In an exemplary method, a sample fluid including the particles may be driven from a sample inlet channel, through a confluence region, and into a sample outlet channel defining a longitudinal axis. Focusing fluid may be introduced into the confluence region from at least two focusing channels along respective introduction axes. Introducing may be rotationally asymmetrical about the longitudinal axis. The introduction axes and the longitudinal axis may collectively extend in three dimensions. The particles may be passed through an interrogation zone of the sample outlet channel. The interrogation zone may be irradiated with light. Optical radiation may be detected from the interrogation zone.
Claims19
A method of detecting particles, the method comprising:
- driving a sample fluid including the particles from a sample inlet channel, through a confluence region, and into a sample outlet channel defining a longitudinal axis;
- introducing focusing fluid into the confluence region from at least two focusing channels along respective introduction axes, wherein introducing is rotationally asymmetrical about the longitudinal axis, and wherein the introduction axes and the longitudinal axis collectively extend in three dimensions;
- passing the particles through an interrogation zone of the sample outlet channel;
- irradiating the interrogation zone with light;
- and
- detecting optical radiation from the interrogation zone.
A method of detecting particles, the method comprising:
- driving a sample fluid including the particles from a sample inlet channel, through a confluence region, and into a sample outlet channel defining a longitudinal axis;
- introducing focusing fluid into the confluence region along two or more introduction axes that collectively have rotational asymmetry about the longitudinal axis;
- passing the particles through an interrogation zone of the sample outlet channel;
- irradiating the interrogation zone with light;
- and
- detecting optical radiation from the interrogation zone.
Dependencies are inferred from the claim text.