Adapter molecule, biomolecule-adapter molecule complex in which said adapter molecule and biomolecule are bound, biomolecule analysis apparatus, and biomolecule analysis method

A method of analyzing a biomolecule, comprising:

• a step of applying a voltage to between a first liquid tank and a second liquid tank, which are placed opposite to each other via a thin film having a nanopore, while filling the first liquid tank with an electrolyte solution containing a bio-adapter molecule complex and filling the second liquid tank with an electrolyte solution to form a potential gradient between the first liquid tank and the second liquid tank with the first liquid tank as a negative or ground potential and the second liquid tank as a positive potential;
• and
• a step of measuring a signal generated when the bio-adapter molecule complex passes through the nanopore from the second liquid tank to the first liquid tank;
• wherein in the step of forming a potential gradient, a single-stranded nucleic acid region of the bio-adapter molecule complex is introduced into the second liquid tank via the nanopore and the bio-adapter molecule complex transfers from the first liquid tank to the second liquid tank by the potential gradient formed between the first liquid tank and the second liquid tank,
• wherein the bio-adapter molecule complex has a molecular motor binding site in a single-stranded nucleic acid region having a 3′ end as a terminal portion thereof and has a primer binding site on the side of the 3′ end relative to the molecular motor binding site,
• the electrolyte solution with which the first liquid tank is filled contains a molecular motor which can bind to the molecular motor binding site,
• the bio-adapter molecule complex has a spacer to which the molecular motor cannot be bound between the molecular motor binding site and the primer binding site,
• the bio-adapter molecule complex has a first block molecule having a diameter larger than the diameter of the nanopore placed in a single-stranded nucleic acid region having a 3′ end as a terminal portion thereof, a second block molecule having a diameter smaller than the diameter of the nanopore binds to a 5′ end of the single-stranded nucleic acid region,
• the single-stranded nucleic acid region having the 5′ end is introduced into the second liquid tank, and the first block molecule placed in the single-stranded nucleic acid region having the 3′ end having the molecular motor binding site is brought into contact with the nanopore to stop the transfer of the bio-adapter molecule complex from the first liquid tank to the second liquid tank.