Systems, compositions, and methods for target entity detection
AssigneeMercy BioAnalytics, Inc.
InventorsJoseph Charles Sedlak, Laura Teresa Bortolin, Daniel Parker Salem
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Abstract
The present disclosure provides technologies for target entity detection. One aspect of the present disclosure provides technologies for detection (e.g., early detection) of a disease, disorder, or condition (e.g., cancer). In another aspect, technologies provided herein are useful for selecting and/or monitoring and/or evaluating efficacy of, a treatment administered to a subject in need thereof, e.g., a subject determined to have or susceptible to cancer. In some embodiments, technologies provided herein are useful for development of companion diagnostics, e.g., by measuring tumor burdens and changes in tumor burdens in conjunction with therapeutics.
Claims10
A method of detecting co-localization of proteins, the method comprising steps of:
- capturing an extracellular vesicle (EV) with a capture agent that binds to a first protein on a surface of the EV;
- contacting the captured EV with (a) a first probe comprising a first oligonucleotide linked to a first ligand that binds to a second protein on the EV surface, wherein the first oligonucleotide comprises a first double-stranded portion and a first single-stranded overhang, and (b) a second probe comprising a second oligonucleotide linked to a second ligand that binds to a third protein on the EV surface, wherein the second oligonucleotide comprises a second double-stranded portion and a second single-stranded overhang, and the overhangs are complementary to each other and can thereby hybridize when the probes are bound to their respective proteins and in proximity, wherein the second and third proteins are different;
- ligating the hybridized first and the second oligonucleotides to form a ligated template;
- and
- measuring co-localization of the second and third proteins on the EV surface by detecting levels of the ligated template.
Dependencies are inferred from the claim text.